Alignment of Penaeidin amino acid sequences and signature determination
Amino acid sequence comparisons were performed to determine a penaeidin signature. From the alignment, consensus sequences of the signal peptide and the two domains were deduced.
1.......10........20........30. .......40........50........60...
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Litvan PEN3-1 MRLVVCLVFLASFALVCQG QVYKGGYTRPIPRPPPFVRPLPGGPIGPYNG-------CPVSCRGISFSQARSCCSRLGRCCHVGKGYSG
Litvan PEN3-2 MRLVVCLVFLASFALVCQG QVYKGGYTRPVPRPPPFVRPLPGGPIGPYNG-------CPVSCRGISFSQARSCCSRLGRCCHVGKGYSG
Litvan PEN3-3 MRLVVCLVFLASFALVCQG QVYKGGYTRPIPRPP-FVRPVPGGPIGPYNG-------CPVSCRGISFSQARSCCSRLGRCCHVGKGYSG
Litvan PEN3-4 MRLVVCLVFLASFALVCQG QVYKGGYTRPIPRPPPFVRPLPGGPIGPYNG-------RPVSCRGISFSQARSCCSRLGRCCHVGKGYSG
Litvan PEN3-5 MRLVVCLVFLAPFALVCHG QVYKGGYTRPIPRPPPFVRPLPGGPIGPYNG-------CPVSCRGISFSQARSCCSRLGRCCHVGKGYSG
Litvan PEN3-6 MRLVVCLVFLASFALVCQG QVYKGGYTRPVPRPP-FVRPLPGGPIGPYNG-------CPVSCRGISFSQARSCCSRLGRCCHVGKGYSG
Litvan PEN3-7 MRLVACLVFLASFALVCQG QVYKGGYTRPIPRPPPFVRPLPGGPIGPYNG-------CPISCRGISFSQARSCCSRLGRCCHVGKGYSG
Litvan PEN3-8 MRLVVCLVFLASFALVCQG QVYKGGYTRPIPRPPPFVRPLPGGPIGPYNG-------CPISCRGISFSQARSCCSRLGRCCHVGKGYSG
Litvan PEN3-9 MRLVVCLVFLASFALVCQG QVYKGGYTRPIPRPPPFVRPLPGGPISPYNG-------CPVSCRGISFSQARSCCSRLGRCCHVGKGYSG
Litvan PEN3-10 MRLVVCLVFLASFALVCQG QVYKGGYTRPIPRPPPFVRPLPGGPIGPYNG-------CPISCRGISFSQARSYCSRLGRCCHVGKGYSG
Litvan PEN3-11 MRLVVCLVFLASFALVCQG QVYKGGYTRPVPRPP-FVRPLPGGPIDPYNG-------CPVSCRGISFSQARSCCSRLGRCCHVGKGYSG
Litsty PEN3-1 MRLVVCLVFLASFALVCQG QGYKGGYTGPVVRP--FVRPIGRPFVTPIGRPVVSGNVCPLSCRGITTLQARSCCSRLGRCCREAKGYSG
Litsty PEN3-2 MRLVVCLVFLASFALVCQG QGYKGGYTGPVVRP--FVRPIGRPVVSYNV--------CPLSCRGITTLQARSCCSRLGRCCREAKGYSG
Litset PEN3-1 MRLVVCLVFLASFALVCQG QGYKGPYTRPILRP--YVRPVVSYNV------------CTLSCRGITTTQARSCCTRLGRCCHVAKGYSG
Litset PEN3-2 MRLVVCLVFLASFALVCQG QGYKGPYTRPILRP--YVRPVVSYNA------------CTLSCRGITTTQARSCSTRLGRCCHVAKGYSG
Litset PEN3-3 MRLVVCLVFLASFALVCQG QGYKGPYTRPILRP--YVRPVVSYNA------------CTLSCRGITTTQARSCCTRLGRCCHVAKGYSG
Litset PEN3-4 MRLVVCLVFLASFALVCQG QGCKGPYTRPILRP--YVRPVVSYNA------------CTLSCRGITTTQARSCCTRLGRCCHVAKGYSG
Penmon PEN3-1 MRLVVCLVFLASFALVCQA QGYQGGYTRPFPRPT-YGGGYHPVPV------------CT-SCHRLSPLQARACCRQLGRCC-DAKQTYG
Penmon PEN3-2 MRLVVCLVFLASFALVCQA QGYQGGYTRPFPRPP-YGGGYHPVPV------------CT-SCHRLSPLQARACCRQLRRCC-DAKQTYG
Penmon PEN3-3 MRLVVCLVFLASFALVCQA QGYQGGYTRPFPRPP-YGGGYHPVPV------------CT-SCHRLSPLQARACCRQLGRCC-DAKQTYG
Fenchi PEN3-1 MRLVVCLVFLASFALVCQG Q--KGGYTRPISRPP-YGGGYGNV--------------CT-SCHVLTTSQARSCCSRFGRCCVPRRGYSG
Pensem PEN3-1 MRLVVCLVLLASLALVCEG QVYQGGYTRPFSRPS-YGGGYVSRPGTV----------CA-SCPVLSSPQARSCCRQLGRCCVR------
Litset PEN4-1 MRLLVCLVFLASFAMVCQG --HSSGYTRPLRKPSRPIFIRPIG--------------CDV-CYGIPSSTARLCCFRYGDCCHLG-----
Litvan PEN4-1 MRLVVCLVFLASFALVCQG --HSSGYTRPLPKPSRPIFIRPIG--------------CDV-CYGIPSSTARLCCFRYGDCCHRG-----
Litvan PEN4-2 MRLVVCLVFLASFALVCQG --YSSGYTRPLPKPSRPIFIRPIG--------------CDV-CYGIPSSTARLCCFRYGDCCHRG-----
Litsch PEN4-1 MRLVVCLVFLASFAMVCQG --HSSGYTRPLPKPSRPIFIRPIG--------------CDV-CYGIPSSTARLCCFRYGDCCHLG-----
Litvan PEN2-1 MRLVVCLVFLASFALVCQG EAYRGGYTGPIPRPPPIGRPPFRPV-------------CNA-CYRLSVSDARNCCIKFGSCCHLVKG---
Litvan PEN2-2 ------------------- --YRGGYTGPIPRPPPIGRPPLRLVV------------C-A-CYRLSVSDARNCCIKFGSCCHLVK----
Litvan PEN2-3 MRLVVCLVFLASFALVCQG EAYRGGYTGPIPRPPPIGRPPLRPV-------------CNA-CYRLSVSDARNCCIKFGSCCHLVKG---
Litsty PEN2-1 MRLVVCLVFLASFALVCQG EAYRGGYTGPIPRPPPYGRPPLGPV-------------CNH-CYRLAFPDARNCCSRFGRCCHLVKG---
Litset PEN2-1 MRLVVCLVFLASFALVCQG GAQRGGFTGPIPRPPPHGRPPLGPI-------------CNA-CYRLSFSDVRICCNFLGKCCHLVKG---
Litsch PEN2-1 MRLVVCLVFLASFALVCQG GAHRGGFTGPIPRPPPHGRPPLGPI-------------CNA-CYRLSFSDVRICCNFLGKCCHLVKG---
Litsch PEN2-2 MRLVVCLVFLASFALVCQG EAQRGGFTGPIPRPPPHGRPPLGPI-------------CNA-CYRLSFSDVRICCNFLGKCCHLVKG---
Farpau PEN2-1 MRLVVCLVFLASFALVCQG HGYKGGYTRPFSRPPFGGIYRPVRPA------------CNA-CYSISFSDALNCCTRFGRCCQIRKG---
Farpau PEN2-2 MRLVVCLVFLASFALVCQG HGYKGGYTRPFSRPPFGGIYGPVRPA------------CNA-CYSISFSDALNCCTRFGRCCQIRKG---
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Penaeidin signature
In red are represented the amino acids conserved in all the penaeidins.Litopenaeus vannamei : Litvan, Litopenaeus setiferus : Litset, Litopenaeus stylirostris : Litsty, Litopenaeus schmitti : Litsch, Penaeus semisulcatus : Pensem, Fenneropenaeus chinensis : Fenchi, Penaeus monodon : Penmon, Farfantepenaeus paulensis : Farpau.
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